2. Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease
  3. Drug Metabolite VD/VDR Endogenous Metabolite
  4. Calcitriol

Calcitriol  (Synonyms: 骨化三醇; 1,25-Dihydroxyvitamin D3)

目录号: HY-10002 纯度: 99.94%
COA 产品使用指南

摩尔计算器

Calcitriol 是活性最大的 vitamin D 代谢物，也是 vitamin D 受体 (VDR) 的激动剂。

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Calcitriol Chemical Structure

Calcitriol Chemical Structure

CAS No. : 32222-06-3

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规格 价格 是否有货 数量
1 mg ￥500
In-stock
2 mg ￥800
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5 mg ￥1500
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10 mg ￥2400
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25 mg ￥4320
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500 mg   询价  

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Customer Review

Other Forms of Calcitriol:

Calcitriol 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 46 篇科研文献

WB
Proliferation Assay

    Calcitriol purchased from MCE. Usage Cited in: Int Immunopharmacol. 2017 Jun;47:182-189.  [Abstract]

    The total number of sneezing and nasal rubbing motions is significantly reduced by 1,25-(OH)2D3, n=9.

    Calcitriol purchased from MCE. Usage Cited in: Oncotarget. 2017 Oct 26;8(59):100187-100195.  [Abstract]

    Calcitriol increases the expressions of JMJD3 and p16INK4A. 786-O cells are treated with vehicle (Veh) or 100nM Calcitriol (Cal) for 48h, and untreated group as control (Con). The expressions of JMJD3 and p16INK4A are determined with western blotting.

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Calcitriol is the most active metabolite of vitamin D and also a vitamin D receptor (VDR) agonist.

    IC50 & Target

    Human Endogenous Metabolite

     

    体外研究
    (In Vitro)

    Calcitriol exerts antiproliferative effects on cervical cancer cells in vitro. Cells decrease by 12.8% when treated with 100 nM Calcitriol for 6 days, compare with control. Inhibition of cell proliferation becomes more pronounced with the increase in Calcitriol concentration. The decrease is 26.1% and 31.6% for 200 and 500 nM Calcitriol, respectively. Treatment with Calcitriol for 72 h induces an evident accumulation of cells in the G1 phase, with approximately 66.18% in 200 nM and 78.10% in 500 nM, compare with the control (24.36%). Calcitriol treatment significantly decreases HCCR-1 protein expression compare with the control in a time- and dose-dependent manner[1]. Calcitriol significantly increases ERα mRNA in a dose dependent manner with an EC50 of 9.8×10-9 M[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Calcitriol 相关抗体:
    体内研究
    (In Vivo)

    Chronic treatment with Calcitriol (150 ng/kg per day for 4.5 months) improves the relaxations (pD2: 6.30±0.09, Emax: 68.6±3.9% in Calcitriol-treated OVX, n=8). Renal blood flow in OVX rats is reduced in both kidneys, and the flow is restored by Calcitriol treatment. The increased expression of COX-2 and Thromboxane-prostanoid (TP) receptor in OVX rat renal arteries is reduced by chronic calcitriol administration[3]. High- and low-dose Calcitriol treatment significantly decreases the systolic blood pressure (SBP) in the fructose-fed rats by 14±4 and 9±4 mmHg, respectively, at Day 56. High-dose Calcitriol treatment (20 ng/kg per day) significantly increases serum ionized calcium level (1.44±0.05 mmol/L) compare with the other groups[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    416.64

    Formula

    C27H44O3
    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    骨化三醇；钙三醇
    结构分类
    初始来源
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    -20°C, protect from light, stored under nitrogen

    *该产品在溶液状态不稳定，建议您现用现配，即刻使用。

    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : 110 mg/mL (264.02 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    Ethanol 中的溶解度 : 100 mg/mL (240.02 mM; 超声助溶)

    H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.4002 mL 12.0009 mL 24.0017 mL
    5 mM 0.4800 mL 2.4002 mL 4.8003 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.75 mg/mL (6.60 mM); 悬浊液; 超声助溶

      此方案可获得 2.75 mg/mL的均匀悬浊液，悬浊液可用于口服和腹腔注射。

      以 1 mL 工作液为例，取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

      生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.75 mg/mL (6.60 mM); 澄清溶液

      此方案可获得 ≥ 2.75 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

      20% SBE-β-CD in Saline 的配制（4°C，储存一周）：2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    请输入您的动物体内配方组成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。

    *该产品在溶液状态不稳定，建议您现用现配，即刻使用。

    您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
    连续给药周期超过半月以上，请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 99.94%

    COA (293 KB) LCMS (95 KB)

    产品使用指南 (1538 KB)
    参考文献
    Cell Assay
    [1]

    HeLa S3 cells are plated at a density of 1,000 cells/well in 96-well plates of Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS), treated with 1% ethanol (control) or various concentrations of Calcitriol (100, 200, and 500 nM) for 72 h. A Cell Counting Kit8 (CCK-8) is used to determine cell proliferation. At 24, 48, 72, 96, 120, and 144 h after culturing with 200 nM Calcitriol, cells are harvested for analysis. Three independent experiments are performed in quadruplicate[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [3]

    Adult female Sprague-Dawley rats weighing 200 to 220g are used in this study. Rats are housed in a temperature-controlled room (~23°C) with a 12-h light/dark cycle. The animals have free access to a standard diet and water. Ovariectomy (OVX) is performed on rats. At 6 months after the surgical procedure, the OVX rats are randomly assigned to either treatment with vehicle dimethyl sulfoxide (OVX+vehicle) or Calcitriol (150 ng/kg daily, OVX+calcitriol). Calcitriol treatment is given by oral gavage and lasted or 4.5 months. Blood pressure and serum Calcitriol level are measured[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    Calcitriol 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 2.4002 mL 12.0009 mL 24.0017 mL 60.0043 mL
    5 mM 0.4800 mL 2.4002 mL 4.8003 mL 12.0009 mL
    10 mM 0.2400 mL 1.2001 mL 2.4002 mL 6.0004 mL
    15 mM 0.1600 mL 0.8001 mL 1.6001 mL 4.0003 mL
    20 mM 0.1200 mL 0.6000 mL 1.2001 mL 3.0002 mL
    25 mM 0.0960 mL 0.4800 mL 0.9601 mL 2.4002 mL
    30 mM 0.0800 mL 0.4000 mL 0.8001 mL 2.0001 mL
    40 mM 0.0600 mL 0.3000 mL 0.6000 mL 1.5001 mL
    50 mM 0.0480 mL 0.2400 mL 0.4800 mL 1.2001 mL
    60 mM 0.0400 mL 0.2000 mL 0.4000 mL 1.0001 mL
    80 mM 0.0300 mL 0.1500 mL 0.3000 mL 0.7501 mL
    100 mM 0.0240 mL 0.1200 mL 0.2400 mL 0.6000 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Calcitriol32222-06-31,25-Dihydroxyvitamin D3骨化三醇钙三醇Drug MetaboliteVD/VDREndogenous MetaboliteVitamin DVitamin D receptorInhibitorinhibitorinhibit

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